BioResearchAgent

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Task T1 · temporal cutoff

Based only on literature published before 2021 in the corpus, what ubiquitin ligase regulates the MDM2-p53 pathway, and how?

Gold evidence

Acceptable answer concepts

  • HERC2
  • ubiquitin ligase
  • regulates MDM2
  • p53 negative feedback loop

Construction note

PMID:31665549 (parsed publication year 2020, safely before the 2021 cutoff) directly answers this from its own title and abstract. Retrieval for this task MUST be restricted to corpus documents with year < 2021 (enforced in code, not just prompt instruction -- see runner.py); nearly all of bra-corpus-v1's other documents are 2025-2026 and must not be retrievable for this task.

How each architecture answered

Baseline 0 · no retrieval

Yuu no Sekai · 622 tokens · 5.2s

(abstained) Based on literature published before 2021, the ubiquitin ligase that regulates the MDM2-p53 pathway is likely to be part of a complex involving E3 ligases such as RNF4 or other members of the SCF (Skp1-Cullin-F-box) family. These ligases can mediate ubiquitylation of MDM2, thereby affecting its stability and function, which in turn influences p53 activity. However, specific mechanisms and direct evidence are not provided in the retrieved evidence.

hypothesisverifiedconf 0.60

The ubiquitin ligase that regulates the MDM2-p53 pathway is likely to be part of a complex involving E3 ligases such as RNF4 or other members of the SCF family.

hypothesisverifiedconf 0.60

These ligases can mediate ubiquitylation of MDM2, thereby affecting its stability and function, which in turn influences p53 activity.

run_failed: 0abstention_correct: 0%concept_coverage: 50%

Baseline 1 · retrieve + synthesize

Yuu no Sekai · 2170 tokens · 6.4s
query(ies): Based only on literature published before 2021 in the corpus, what ubiquitin ligase regulates the MDM2-p53 pathway, and how?

The ubiquitin E3 ligase MDM2 regulates the MDM2-p53 pathway by forming a complex with oligomeric p53, HERC2, and NEURL4. HERC2 knockdown reduces MDM2 protein levels by inhibiting its mRNA expression, and DNA damage dissociates MDM2 from the complex, increasing phosphorylation and acetylation of p53 bound to HERC2 and NEURL4.

factualverifiedconf 0.95

MDM2 forms a complex with oligomeric p53, HERC2, and NEURL4.

PMID:31665549Direct support
factualverifiedconf 0.95

HERC2 knockdown results in a decline in MDM2 protein levels without affecting its stability.

PMID:31665549Direct support
factualverifiedconf 0.95

DNA damage induces dissociation of MDM2 from the p53/HERC2/NEURL4 complex.

PMID:31665549Direct support
factualverifiedconf 0.95

MDM2 promoter competes with HERC2 for binding of oligomeric, phosphorylated, and acetylated p53.

PMID:31665549Direct support
run_failed: 0retrieval_recall_at_k: 100%retrieval_mrr: 100%claim_support_rate: 100%unsupported_claim_rate: 0%citation_precision: 100%source_validity_rate: 100%abstention_correct: 100%concept_coverage: 75%

Agent 1 · planner + retrieve + synthesize

Yuu no Sekai · 2414 tokens · 8.2s
query(ies): ubiquitin ligase regulates MDM2-p53 pathway before 2021 | mechanism of ubiquitin ligase action on MDM2-p53 pathway pre-2021
planner → {"queries": ["ubiquitin ligase regulates MDM2-p53 pathway before 2021", "mechanism of ubiquitin ligase action on MDM2-p53 pathway pre-2021"]}

The ubiquitin E3 ligase MDM2 regulates the MDM2-p53 pathway by forming a complex with oligomeric p53, HERC2, and NEURL4. HERC2 knockdown reduces MDM2 protein levels by inhibiting its mRNA expression, and DNA damage dissociates MDM2 from the complex, increasing phosphorylation and acetylation of p53 bound to HERC2 and NEURL4.

factualverifiedconf 0.95

MDM2 forms a complex with oligomeric p53, HERC2, and NEURL4.

PMID:31665549Direct support
factualverifiedconf 0.95

HERC2 knockdown results in a decline in MDM2 protein levels without affecting its stability.

PMID:31665549Direct support
factualverifiedconf 0.95

DNA damage induces dissociation of MDM2 from the p53/HERC2/NEURL4 complex.

PMID:31665549Direct support
factualverifiedconf 0.95

MDM2 promoter competes with HERC2 for binding of oligomeric, phosphorylated, and acetylated p53.

PMID:31665549Direct support
run_failed: 0retrieval_recall_at_k: 100%retrieval_mrr: 100%claim_support_rate: 100%unsupported_claim_rate: 0%citation_precision: 100%source_validity_rate: 100%abstention_correct: 100%concept_coverage: 75%